Effect of Different Cytokinins on Shoot Outgrowth and Bioactive Compounds Profile of Lemograss Essential Oil

Lemongrass (Cymbopogon citratus) essential oil (EO) is a major source of bioactive compounds (BC) with anticancer activity such as ?-citral, limonene, geraniol, geranyl acetate, and ?-caryophyllene. Comparative studies about cytokinin effects on BC profiles in lemongrass are missing. Here, we evaluated four cytokinins (2iP, tZ, BAP, KIN) two different osmotic media, MS-N (3% sucrose, 3 g L?1 Gelrite™) MS-S (5% 5 Gelrite™). It results higher multiplication rate BAP containing medium compared to KIN, 2iP (p ? 0.05). While shoots grown MS-N/BAP, KIN exhibited highly branching morphology, MS-N/2iP produced less architecture. profile analysis established plants pots revealed that their maxima production depends the vitro shoot growth conditions: i.e., highest content (80%) ?-citral were cultured MS-S/BAP 0.05), limonene (41%) MS-N/2iP, or acetate (25.79%) MS-S/2iP. These indicate it possible increase address by manipulating type pressure culture media. The protocol described here currently successfully applied for somatic embryogenesis induction genetic transformation lemongrass.